Npuc18 vector map pdf

For cloning vector part7 and binary vector part27 see. To the extent possible under law, uploaders on this site have waived all to their vector images. See vector map perform a cotransfection of the recombinant plasmid and bd baculogold linearized baculovirus dna cat. These changes together result in a temperaturedependent copy number of. The linearized ptg19t vector with 3dt overhangs prevent vector recircularization, therefore resulting in high percentage of recombinant clones and low background. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the nterminal secretion signal. Expression of target dna is enabled by the presence of a. The puc18 puc19 sequence is stored as a pdf file on the genaxxon webpage. This is a free resource for the scientific community that is compiled by addgene.

A repository of over 200,000 plasmids including protein structure initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms. Description the pgl4 luciferase reporter vectorsae are the next generation of reporter gene vectors optimized for expression in mammalian cells. Atg start codon present on the pet translation vectors. The cut sites for some restriction enzyme are indicated on the plasmid. Map of panama single color is a fully layered, editable vector map file. It contains identical multiple cloning site mcs as puc19 vector except that it is arranged in opposite orientation. The puc18 and puc19 plasmids enable successful cloning of large dna fragments larger than those cloned with a m mp18 rf phage vector. Description the ptg19t vector is designed for rapid and efficient cloning of pcr products with 3da overhangs. Thermo scientific puc19 vector is a small, high copy number, e. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.

The expression efficiency of recombinant human tumor necrosis factor. Map of the glutathione stransferase fusion vectors showing the reading frames and main features. A vector file ai, pdf has flexible tools for displaying elements of maps you can discrete or arbitrary scaling map performing group operation with selected features. The cloningexpression region of the coding strand transcribed by t7 rna polymerase is shown below. Terms and conditions 20042018 harvard medical school plasmid was created and is maintained by the dfhcc dna resource core at harvard medical schooldfhcc dna resource. Confirm sequencing primer sequences match vector before sequencing. The ptz57rt vector was obtained from mbi fermentas. Novel highlevel constitutive expression system, phce vector. Even though stop codons in all three frames are not depicted in this map, all thirteen vectors have stop codons in all three frames downstream from the multiple cloning site.

Contour map of the middle east in vector format, illustrator and svg. To see this sequence with restriction sites, features. It contains the pmb1 origin of replication from pbr322, but it lacks the rop gene and carries a point mutation in the rnaii transcript g 2975 in pbr322 to a 8 in puc19. Plasmid puc57, 2710 bp in length, is a derivative of puc19. These cloning vectors contain a multiple cloning site at the lacz region that enables recombinant plamids to be verified via bluewhite colony screening using agar plates containing iptg and xgal.

The vector contains two multiple cloning sites mcs, each of which is preceded by a t7 promoterlac operator and a ribosome binding site rbs. The artificial plasmid puc18 has been genetically engineered to include 1 a gene for antibiotic resistance to ampicillin amp r, and 2 a gene and its promoter for the enzyme betagalactosidase lacz. The seeds were germinated in plastic pots containing garden soil and the plants were grown in natural conditions. The exact position of genetic elements is shown on the map termination codons included. Biodefense and emerging infections research resources repository bei resources is the leading source for reagents, tools and information for studying niaids category a, b, and c priority pathogens and emerging infectious disease agents. The pcr amplified products were prepared with a overhang and cloned into the ptz57rt vectors adopting manufacturers protocols mbi fermentas, hanover, md, usa.

Highlights purified by chromatography using proprietary patented technology more than. You are free to edit, distribute and use the images for unlimited commercial purposes without asking permission. New vectors for high level expression of recombinant proteins. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the circular map. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. It contains identical multiple cloning site mcs as puc18 vector except that it is arranged in opposite orientation. Gleave ap 1992 a versatile binary vector system with a tdna organisational structure conducive to efficient integration of cloned dna into the plant genome. The map, notes, and annotations on this page and in the sequence map file are ed material. Thermo scientific puc18 vector is a small, high copy number, e. A plasmid is a circular dsdna molecule a few hundred or thousand base pairs in circumference.

The molecule is a small doublestranded circle, 2686 base pairs in length, and has a high copy number. Free vector world maps available in adobe illustrator, eps, pdf, png and jpg formats to download. Vector map of panama single color free vector maps. This vector is designed for cloning and generation of exoiii deletions. Click on the button download pdfsvg and look for the downloaded file in your downloads folder. The vector also carries the pbr322derived cole1 replicon, laci gene and ampicillin resistance gene. This listings of restriction sites for the pgl2 luciferase reporter vectors are provided in sections 7. Note the specific antibiotic to be used with this vector. New vectors for high level expression of recombinant proteins in bacteria david j. The coordinates refer to the position of the first nucleotide in each. Dixon department of biological chemistry, university of michigan medical school, ann arbor, michigan 481090606 received october 4,1991 a system has been developed for synthesis and rapid. Naturallyoccurring plasmids are viruses of bacteria.